Pseudomonas luteola lipase: a new member of the 320-residue Pseudomonas lipase family

@inproceedings{Litthauer2002PseudomonasLL,
  title={Pseudomonas luteola lipase: a new member of the 320-residue Pseudomonas lipase family},
  author={Derek Litthauer and Anne Ginster and Etienne van Eeden Skein},
  year={2002}
}
An extracellular lipase from Pseudomonas luteola was purified 17 fold with 16% recovery by two-phase partitioning, anion exchange and exclusion chromatography. The purified enzyme is relatively thermostable with a half life of 116 min at 65°C and an optimal temperature of 55°C. The enzyme is unstable at pH 1 but at pH12.25 the half life is 84 min. A preference for medium chain saturated and unsaturated fatty acids was shown while the position and configuration of the acyl double bond had no… CONTINUE READING

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