Proximity of the start codon to a leaderless mRNA's 5' terminus is a strong positive determinant of ribosome binding and expression in Escherichia coli.

Abstract

An AUG start codon is an important determinant of ribosome binding and expression of leaderless mRNAs in Escherichia coli. Using reporter constructs encoding mRNAs where the AUG start codon is preceded by untranslated leaders of various length and sequence, we find that close proximity of the start codon to the 5' terminus and the leader sequence are strong… (More)
DOI: 10.1128/JB.00756-10

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@article{Krishnan2010ProximityOT, title={Proximity of the start codon to a leaderless mRNA's 5' terminus is a strong positive determinant of ribosome binding and expression in Escherichia coli.}, author={Karthik Krishnan and William J Van Etten and Gary R. Janssen}, journal={Journal of bacteriology}, year={2010}, volume={192 24}, pages={6482-5} }