Prox1 Is a Novel Coregulator of Ff1b and Is Involved in the Embryonic Development of the Zebra Fish Interrenal Primordium

  title={Prox1 Is a Novel Coregulator of Ff1b and Is Involved in the Embryonic Development of the Zebra Fish Interrenal Primordium},
  author={Yi-Wen Liu and Wei Gao and Hui-Ling Teh and J Tan and Woon Khiong Chan},
  journal={Molecular and Cellular Biology},
  pages={7243 - 7255}
ABSTRACT Steroidogenic factor 1 (SF-1) plays an essential role in adrenal development, although the exact molecular mechanisms are unclear. Our previous work established that Ff1b is the zebra fish homologue of SF-1 and that its disruption by antisense morpholinos leads to a complete ablation of the interrenal organ. In this study, results of biochemical analyses suggest that Ff1b and other Ff1 members interact with Prox1, a homeodomain protein. Fine mapping using site-directed mutants showed… 

Novel steroidogenic factor‐1 homolog (ff1d) is coexpressed with anti‐Mullerian hormone (AMH) in zebrafish

Comparison of sequences and expression patterns indicate that ff1d is a new candidate for sex determination and differentiation in a way similar to SF‐1, possibly involving AMH.

A Prospero-related Homeodomain Protein Is a Novel Co-regulator of Hepatocyte Nuclear Factor 4α That Regulates the Cholesterol 7α-Hydroxylase Gene*

Results suggest that Prox1 is a novel co-regulator of HNF4α that may play a key role in the regulation of bile acid synthesis and gluconeogenesis in the liver.

Prospero-related homeobox (Prox1) is a corepressor of human liver receptor homolog-1 and suppresses the transcription of the cholesterol 7-alpha-hydroxylase gene.

Prox1, a prospero-related homeobox transcription factor, identified through a yeast two-hybrid screening, can directly interact with human LRH-1 (hLRH- 1) and suppresses hLRh-1-mediated transcriptional activation of human cyp7a1 gene.

Characterization of a novel prospero-related homeobox gene, Prox2

It is demonstrated that mouse Prox2 is dispensable for embryonic development, horizontal cell generation and fertility in contrast to mouseprospero-related homeobox genes.

PCNA interacts with Prox1 and represses its transcriptional activity

Data suggest that Prox1-PCNA interactions may in part prevent the activation of βB1-crystallin expression in the lens epithelium, where Prox 1 expression is high and PCNA levels are low.

Mafs, Prox1, and Pax6 Can Regulate Chicken βB1-Crystallin Gene Expression*

It is proposed that Pax6 occupies and represses the chicken βB1-crystallin promoter in lens epithelial cells, and is displaced by Prox1 and Mafs, which activate the promoter, in differentiating cortical fiber cells.

Functional conservation of interactions between a homeodomain cofactor and a mammalian FTZ‐F1 homologue

This study has identified and characterized the homeodomain protein Prox1 as a co‐repressor for liver receptor homologue 1 (LRH1/NR5A2), a master regulator of cholesterol homeostasis in mammals and suggests that interactions between LRH1 and ProX1 may fulfil roles both during development of the enterohepatic system and in adult physiology of the liver.

Pituitary-interrenal interaction in zebrafish interrenal organ development.

A gradual transition from early pituitary-independent interrenal organogenesis to developmental control by the anterior domain of pituitARY corticotrophs acting via Mc2 receptors is demonstrated.

Molecular Cloning and Expression Characterization of Prox1 in Goldfish ( Carassius auratus) Eye Development

The results suggest that the Prox1 gene plays an important role during lens development, and the roles are different at different developmental stages.



A Zebrafish Ftz-F1 (Fushi Tarazu Factor 1) Homologue Requires Multiple Subdomains in the D and E Regions for Its Transcriptional Activity*

It is suggested that the D and E regions of zFF1A possess both positive and negative transactivation functions, and interdomain “cross-talk” may confer the full transcriptional activity of the protein.

Steroidogenic factor 1 and Dax-1 colocalize in multiple cell lineages: potential links in endocrine development.

The striking colocalization of SF-1 and Dax-1 supports the model that they are intimately linked in a common pathway of endocrine development.

Dax1 expression is dependent on steroidogenic factor 1 in the developing gonad.

The results show that SF1 consensus-binding sites close to the start of Dax1 transcription are important in regulating levels of expression in the developing gonad and provide novel information on how a specific enhancer element acts in different cell types at different stages of development.

Zebrafish ftz-f1 gene has two promoters, is alternatively spliced, and is expressed in digestive organs.

R reverse transcriptase-PCR and in situ hybridization experiments showed that the ff1 transcript is expressed in the hypothalamus, spinal cord, mandibular arch and digestive organs, including pancreas, liver, and intestine, implying its function in gut development.

Direct Interaction of SRY-Related Protein SOX9 and Steroidogenic Factor 1 Regulates Transcription of the Human Anti-Müllerian Hormone Gene

SOX9 is identified as an interaction partner of SF-1 that could be involved in the Sertoli cell-specific expression of AMH during embryogenesis and is proposed to be a combinatorial protein-protein and protein-DNA complex.

Developmental defects of the ventromedial hypothalamic nucleus and pituitary gonadotroph in the Ftz‐F1 disrupted mice

  • K. ShinodaHong Lei E. Li
  • Biology
    Developmental dynamics : an official publication of the American Association of Anatomists
  • 1995
The present results clearly showed that the hypothalamic and pituitary Ad4BPs are essential to normal development of the functional VMH and gonadotroph through some mechanism distinct from that in the peripheral steroidogenic tissues.

Autoregulatory Loop in the Regulation of the Mammalian ftz-f1 Gene (*)

The mammalian ftz-f1 (mftz-f1) gene encoding Ad4BP/SF-1 has been demonstrated to be essential for the development of adrenal and gonadal glands. In a previous study, we identified an E box as the

Antagonistic action of Six3 and Prox1 at the γ-crystallin promoter

Cryg expression increases as Six3 expression decreases during ongoing lens development, and expression of Crygd/e/f is predicted to remain low at this time, while Prox1 and Six3 are present at the beginning of lens development.

Segmentation gene product Fushi tarazu is an LXXLL motif-dependent coactivator for orphan receptor FTZ-F1

It is proposed that FTZ may represent a category of LXXLL motif-dependent coactivators for nuclear receptors and that direct interaction is required for regulation of gene expression by FTZ-F1.

Antagonistic action of Six3 and Prox1 at the gamma-crystallin promoter.

Since Prox1 and Six3 are present at the beginning of lens development, expression of CRYGD/e/f is predicted to remain low at this time, and cell transfection experiments indicated that CRYG expression increases as Six3 expression decreases during ongoing lens development.