Protoplast production fromPorphyra linearis using a simplified agarase procedure capable of commercial application

@article{Chen2004ProtoplastPF,
  title={Protoplast production fromPorphyra linearis using a simplified agarase procedure capable of commercial application},
  author={Lin Chen and James S. Craigie and Zhuohong Kenny Xie},
  journal={Journal of Applied Phycology},
  year={2004},
  volume={6},
  pages={35-39}
}
Abalone enzymes, Cellulase R-10, Macerozyme and agarase fromPseudomonas atlantica were tested for activity on agarose, cellulose, xylan, the cell wall matrix and porphyran isolated fromPorphyra linearis. Agarase, and to a lesser extent Macerozyme, digested both agarose and porphyran. Abalone enzymes and Cellulase R-10 reacted only weakly with porphyran. A simple standardized protocol for making protoplasts fromPorphyra linearis was developed using 0.025% agarase in seawater without added… CONTINUE READING