Corpus ID: 88069375

Protoplast preparation and regeneration of Aureobasidium pullulans

  title={Protoplast preparation and regeneration of Aureobasidium pullulans},
  author={Hu Yao-hui},
  journal={Food Science and Technology International},
  • Hu Yao-hui
  • Published 2008
  • Chemistry
  • Food Science and Technology International
The effect factors such as enzyme system,mycelium age,temperature and time of enzyme treatment on the formation and regeneration of protoplasts in Aureobasidium pullulans were studied.The results show that the suitable conditions are enzyme concentration 5 mg/mL(snail enzyme,cellulose),my-celium age 24 h,zymolytic temperature 37 ℃,time 1 h.The protoplast formation rate is 100%,The regeneration rate is 64.6%.