Protoplast formation and regeneration in Streptomyces flavopersicus.

@article{Lyutzkanova1993ProtoplastFA,
  title={Protoplast formation and regeneration in Streptomyces flavopersicus.},
  author={D Lyutzkanova and Biliana Nikolova and M Stoilova-Discheva and T S Todorov},
  journal={Letters in applied microbiology},
  year={1993},
  volume={16 4},
  pages={217-9}
}
Protoplasts of Streptomyces flavopersicus with the highest regeneration frequency were isolated from late log phase mycelium grown in a two-stage culture system with 2% glycine in the medium. Of the 11 regeneration media tested, R9 was selected as the most efficient with 29 degrees C as the best temperature.