Protoplast culture and plant regeneration ofPinellia ternata

@article{He2005ProtoplastCA,
  title={Protoplast culture and plant regeneration ofPinellia ternata},
  author={Yikun He and Changfu Zhu and M. Y. He and Shui Hao},
  journal={Plant Cell Reports},
  year={2005},
  volume={16},
  pages={92-96}
}
A study was undertaken to develop a protoplast regeneration system for pinellia. A yield of 19 29 x 105 protoplasts/g F. W. could be obtained from cell suspension cultures incubated in a digestion enzyme solution with 2% cellulase Onzuka R-10, 10% pectinase (Sigma), 0.01% pectolyase Y23. K8P and modified MS media were used to culture protoplasts in: a) liquid, b) liquid-solid double layer, or c) agarose embedded protoplast culture. The former two were conducive to colony formation from… Expand
Somatic embryogenesis and plant regeneration in leaf and petiole explant cultures and cell suspension cultures of Pinellia tripartita
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Culture conditions for high frequency plant regeneration via somatic embryogenesis and from cell suspension cultures of Pinellia tripartita are described and regenerated plantlets developed to maturity in a growth chamber. Expand
EFFICIENT IN VITRO PLANT REGENERATION OF PINELLIA TERNATA (THUNB) BREIT
TLDR
Establishment of an efficient plant regeneration procedure provides a basis for rapid in vitro propagation of P. ternata, which is in great demand in China, and represents an excellent tool for further work on its genetic manipulation. Expand
Mass propagation of microtubers from suspension cultures of Pinellia ternata cells and quantitative analysis of succinic acid in Pinellia tubers
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The in vitro P. ternata microtuber proliferation system established in this study is thus an efficient alternative for the mass production of medicinal tubers. Expand
Establishment of high frequency plant regeneration system from leaf explants of Pinellia koreana via bulblets formation
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Culture conditions for high frequency plant regeneration via bulblet formation from leaf explant cultures of Pinellia koreana are described, which will be useful for mass propagation and long-term preservation of genetic resources of P. koreANA. Expand
Effect of enzyme concentrations on protoplast isolation and protoplast culture of Spathiphyllum and Anthurium
TLDR
Vital protoplasts from Spathiphyllum wallisii ‘Alain’ and Anthurium scherzerianum ‘238’ were isolated from both somatic embryos and leaves and development until colony stage was achieved using agarose beads for protoplast culture. Expand
Induction and in vitro alkaloid yield of calluses and protocorm-like bodies (PLBs) from Pinellia ternata
TLDR
The induction of calluses and PLBs with alkaloid content greater than that of field-grown tubers indicates the potential use of these tissue culture materials for bioprocessing alkaloids from P. ternata and for the study of alkaloidal metabolism. Expand
Induction and in vitro alkaloid yield of calluses and protocorm-like bodies (PLBs) from Pinellia ternata
This study investigated the induction and in vitro alkaloid yield of calluses and protocorm-like bodies (PLBs) from Pinellia ternata (Thunb.) Berit (Araceae). We planned to use this material inExpand
Rapid Propagation of the Medicinal Plant Pinellia ternata by in vitro Leaves Culture
PENG, Z. S., C. K. LUO, P. CAI, Z. C. MAO, C. Y. KANG and J. YANG, 2007. Rapid propagation of the medicinal plant Pinellia ternata by in vitro leaves culture. Bulg. J. Agric. Sci., 12: 01-06 PinelliaExpand
Mass production of Pinellia ternata multiple egg-shaped micro-tubers (MESMT) through optimized growth conditions for use in ethnomedicine
TLDR
This is the first study to report on the optimal conditions for in vitro propagation of Pinellia ternata multiple egg-shaped micro-tubers and provides an invaluable basis for its large-scale production to meet its demand in traditional medicine. Expand
Accumulation of salicylic acid-elicited alkaloid compounds in in vitro cultured Pinellia ternata microtubers and expression profiling of genes associated with benzoic acid-derived alkaloid biosynthesis
TLDR
It is determined that SA-promoted accumulation of alkaloids is associated with genes in the benzoic acid and alkaloid derivative biosynthesis pathway in P. ternata microtubers cultured in vitro without addition of exogenous plant growth regulators. Expand
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