Protoplast culture and fusion between Brassica carinata and Brassica napus.

@inproceedings{Klma2009ProtoplastCA,
  title={Protoplast culture and fusion between Brassica carinata and Brassica napus.},
  author={Miroslav Kl{\'i}ma and Eyasu Abraha and Miroslava Vyvadilov{\'a} and Miroslav Bechyne},
  year={2009}
}
In this study, attempts were made to select in vitro responsible genotypes and to fuse the isolated protoplasts of Brassica carinata and Brassica napus breeding lines (BC DH Dodolla, BC DH -1, BC DH-6 and BN OP-1, BN-SL03/04), obtained from our previous experiments. Combination of three different PEG concentrations (20%, 25% or 30%) and two different treatment durations (15 and 20 min.) were tested. Our experiments identified several genotypes (Brassica carinata DH BC-6 and DH BC-1, Brassica… CONTINUE READING

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