Proton transport through influenza A virus M2 protein reconstituted in vesicles.

@article{Moffat2008ProtonTT,
  title={Proton transport through influenza A virus M2 protein reconstituted in vesicles.},
  author={James Moffat and Viksita Vijayvergiya and Philip Fei Gao and Timothy A. Cross and Dixon J. Woodbury and David D. Busath},
  journal={Biophysical journal},
  year={2008},
  volume={94 2},
  pages={434-45}
}
Influenza A virus M2 protein is known to form acid-activated, proton-selective, amantadine-sensitive channels. We directly measured proton uptake in vesicles containing reconstituted M2 by monitoring external pH after addition of valinomycin to vesicles with 100-fold-diluted external [K(+)]. External pH typically increased by a few tenths of a pH unit over a few minutes after valinomycin addition, but proton uptake was not significantly altered by acidification. Under neutral conditions… CONTINUE READING