Proton translocation by bacteriorhodopsin in the absence of substantial conformational changes.

@article{Tittor2002ProtonTB,
  title={Proton translocation by bacteriorhodopsin in the absence of substantial conformational changes.},
  author={Joerg Tittor and Stefan Paula and Srisunder Subramaniam and Joachim Heberle and Richard Henderson and Dieter Oesterhelt},
  journal={Journal of molecular biology},
  year={2002},
  volume={319 2},
  pages={555-65}
}
Unlike wild-type bacteriorhodopsin (BR), the BR triple mutant D96G/F171C/F219L has been shown to undergo only minor structural rearrangements during its photocycle. Nonetheless, the mutant is capable of transporting protons at a rate of 125(+/-40) H+/BR per minute under light-saturating conditions. Light adaptation of the triple mutant's retinal proceeds in a pH-dependent manner up to a maximum of 63% all-trans. These two findings imply that the transport activity of the triple mutant comprises… CONTINUE READING

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