Proton sponge trick for pH-sensitive disassembly of polyethylenimine-based siRNA delivery systems.

@article{Creusat2010ProtonST,
  title={Proton sponge trick for pH-sensitive disassembly of polyethylenimine-based siRNA delivery systems.},
  author={Gaelle Creusat and A. Rinaldi and Etienne Weiss and Rkia Elbaghdadi and J G R{\'e}my and Rita Mulherkar and Guy Zuber},
  journal={Bioconjugate chemistry},
  year={2010},
  volume={21 5},
  pages={994-1002}
}
Small interfering RNAs offer novel opportunities to inhibit gene expression in a highly selective and efficient manner but depend on cytosolic translocation with synthetic delivery systems. The polyethylenimine (PEI) is widely used for plasmid DNA transfection. However, the water-soluble PEI does not form siRNA polyplexes stable enough in extracellular media for effective delivery. We previously showed that rendering PEI insoluble in physiological media, without modifying drastically its… CONTINUE READING