Proton leak and CFTR in regulation of Golgi pH in respiratory epithelial cells.

@article{Chandy2001ProtonLA,
  title={Proton leak and CFTR in regulation of Golgi pH in respiratory epithelial cells.},
  author={Grischa Chandy and Michael Grabe and Harry P. Moore and Terry E. Machen},
  journal={American journal of physiology. Cell physiology},
  year={2001},
  volume={281 3},
  pages={
          C908-21
        }
}
Work addressing whether cystic fibrosis transmembrane conductance regulator (CFTR) plays a role in regulating organelle pH has remained inconclusive. We engineered a pH-sensitive excitation ratiometric green fluorescent protein (pHERP) and targeted it to the Golgi with sialyltransferase (ST). As determined by ratiometric imaging of cells expressing ST-pHERP, Golgi pH (pH(G)) of HeLa cells was 6.4, while pH(G) of mutant (DeltaF508) and wild-type CFTR-expressing (WT-CFTR) respiratory epithelia… CONTINUE READING

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References

Publications referenced by this paper.
SHOWING 1-10 OF 63 REFERENCES

The mechanochemistry of V-ATPase proton pumps.

  • Biophysical journal
  • 2000
VIEW 13 EXCERPTS
HIGHLY INFLUENTIAL

Determinants of the pH of the Golgi complex.

  • The Journal of biological chemistry
  • 2000
VIEW 14 EXCERPTS
HIGHLY INFLUENTIAL

Golgi Alkalinization by the Papillomavirus E5 Oncoprotein

  • The Journal of cell biology
  • 2000
VIEW 4 EXCERPTS
HIGHLY INFLUENTIAL

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