Proton gradient formation in early endosomes from proximal tubules.

  title={Proton gradient formation in early endosomes from proximal tubules.},
  author={Vladimir Marshansky and Patrick Vinay},
  journal={Biochimica et biophysica acta},
  volume={1284 2},
Heavy endosomes were isolated from proximal tubules using a combination of magnesium precipitation and wheat-germ agglutinin negative selection techniques. Two small GTPases (Rab4 and Rab5) known to be specifically present in early endosomes were identified in our preparations. Endosomal acidification was followed fluorimetrically using acridine orange. In presence of chloride ions and ATP, the formation of a proton gradient (delta pH) was observed. This process is due to the activity of an… CONTINUE READING


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