Proton and fluorine nuclear magnetic resonance spectroscopic observation of hemiacetal formation between N-acyl-p-fluorophenylalaninals and alpha-chymotrypsin.


Proton nuclear magnetic resonance (NMR) spectroscopy shows that the free aldehyde and not the hydrate of N-acetyl-DL-p-fluorophenylalaninal binds to alpha-chymotrypsin. A proton NMR cross-saturation experiment shows that the initial noncovalent complex is in equilibrium with a hemiacetal formed between the aldehyde and the active site serine residue… (More)


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