Proton Nuclear Magnetic Resonance Study of Hirudin: Resonance Assignment and Secondary Structure?

Abstract

The 'H NMR spectrum of the 65-residue protein hirudin is assigned in a sequential manner by using a combination of two-dimensional nuclear magnetic resonance techniques to demonstrate through-bond and through-space (<5-i() connectivities. The secondary structure of hirudin is deduced from a qualitative interpretation of the nuclear Overhauser effects… (More)

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