Protocols to detect senescence-associated beta-galactosidase (SA-βgal) activity, a biomarker of senescent cells in culture and in vivo

@article{DebacqChainiaux2009ProtocolsTD,
  title={Protocols to detect senescence-associated beta-galactosidase (SA-βgal) activity, a biomarker of senescent cells in culture and in vivo},
  author={Florence Debacq-Chainiaux and Jorge D. Erusalimsky and Judith Campisi and Olivier Toussaint},
  journal={Nature Protocols},
  year={2009},
  volume={4},
  pages={1798-1806}
}
Normal cells can permanently lose the ability to proliferate when challenged by potentially oncogenic stress, a process termed cellular senescence. Senescence-associated beta-galactosidase (SA-βgal) activity, detectable at pH 6.0, permits the identification of senescent cells in culture and mammalian tissues. Here we describe first a cytochemical protocol suitable for the histochemical detection of individual senescent cells both in culture and tissue biopsies. The second method is based on the… CONTINUE READING
Highly Influential
This paper has highly influenced 20 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS

Citations

Publications citing this paper.
Showing 1-10 of 322 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 42 references

lysosomal dysfunction and ganglioside accumulation in endothelial cells

  • Patschan, S. et al. Mapping mechanisms, charting the time course of premature cell senescence, apoptosis
  • Am. J. Physiol. Renal Physiol. 294, F100–F109
  • 2008

Similar Papers

Loading similar papers…