Protocols for lysine conjugation.

@article{Brun2013ProtocolsFL,
  title={Protocols for lysine conjugation.},
  author={Marie-Priscille Brun and Laurence Gauzy-Lazo},
  journal={Methods in molecular biology},
  year={2013},
  volume={1045},
  pages={173-87}
}
Currently, the most widely used chemical methodology for the conjugation of drugs to monoclonal antibodies involves either lysine or cysteine residues. In this chapter, several methods for the preparation of antibody-drug conjugates (ADCs) through conjugation of drugs to solvent-exposed ε-amino groups of lysine residues are described. These methods apply to various cytotoxic agents, both tubulin binders and DNA-targeting agents and different types of linkers, cleavable or not, peptidic or… CONTINUE READING