Protocol for high-sensitivity/long linear-range spectrofluorimetric DNA quantification using ethidium bromide.

@article{Bonasera2007ProtocolFH,
  title={Protocol for high-sensitivity/long linear-range spectrofluorimetric DNA quantification using ethidium bromide.},
  author={Veronica Bonasera and Saverio Alberti and Andrea Sacchetti},
  journal={BioTechniques},
  year={2007},
  volume={43 2},
  pages={
          173-4, 176
        }
}
Ethidium bromide (EtBr) is the most widely used fluorescent dye in nucleic acid gel electrophoresis since decades. However it has been essentially forgotten in DNA quantification by spectrofluorimetry. While investigating sensitivity and dynamic range of available fluorochromes, we found that EtBr permits much more sensitive fluorimetric measurements than previously thought. We report here a revised, accurate, and easy-to-use protocol for EtBr-based DNA quantification in solution, which… CONTINUE READING

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