Protocol: Streamline cloning of genes into binary vectors in Agrobacterium via the Gateway® TOPO vector system

@article{Xu2007ProtocolSC,
  title={Protocol: Streamline cloning of genes into binary vectors in Agrobacterium via the Gateway® TOPO vector system},
  author={Ruqiang Xu and Qingshun Quinn Li},
  journal={Plant Methods},
  year={2007},
  volume={4},
  pages={4 - 4}
}
In plant functional genomic studies, gene cloning into binary vectors for plant transformation is a routine procedure. Traditionally, gene cloning has relied on restriction enzyme digestion and ligation. In recent years, however, Gateway® cloning technology (Invitrogen Co.) has developed a fast and reliable alternative cloning methodology which uses a phage recombination strategy. While many Gateway- compatible vectors are available, we frequently encounter problems in which antibiotic… CONTINUE READING

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