Proto-oncogene expression during early myeloid differentiation of normal peripheral blood progenitor cells.

Peripheral blood mononuclear cells cultured in liquid medium with fetal calf serum undergo differentiation to myeloblasts (7 days) and then mature granulocytes (14-21 days). This culture system was used to study proto-oncogene expression during pre-myeloblast myeloid differentiation. c-myc mRNA was present in the peripheral blood mononuclear cells placed… (More)