Proteorhodopsin is a light-driven proton pump with variable vectoriality.

  title={Proteorhodopsin is a light-driven proton pump with variable vectoriality.},
  author={Thomas Friedrich and Sven Geibel and Rolf Kalmbach and I. V. Chizhov and Kenichi Ataka and Joachim Heberle and Martin Engelhard and Ernst Bamberg},
  journal={Journal of molecular biology},
  volume={321 5},
Proteorhodopsin, a homologue of archaeal bacteriorhodopsin (BR), belongs to a newly identified family of retinal proteins from marine bacteria, which could play an important role in the energy balance of the biosphere. We cloned the cDNA sequence of proteorhodopsin by chemical gene synthesis, expressed the protein in Escherichia coli cells, purified and reconstituted the protein in its functional active state. The photocycle characteristics were determined by time-resolved absorption and… CONTINUE READING