Proteomics strategy for quantitative protein interaction profiling in cell extracts

  title={Proteomics strategy for quantitative protein interaction profiling in cell extracts},
  author={Kirti Sharma and Christoph Weber and Michaela Bairlein and Zolt{\'a}n Greff and Gy{\"o}rgy K{\'e}ri and Juergen Cox and Jesper V. Olsen and Henrik Daub},
  journal={Nature Methods},
We report a proteomics strategy to both identify and quantify cellular target protein interactions with externally introduced ligands. We determined dissociation constants for target proteins interacting with the ligand of interest by combining quantitative mass spectrometry with a defined set of affinity purification experiments. We demonstrate the general utility of this methodology in interaction studies involving small-molecule kinase inhibitors, a tyrosine-phosphorylated peptide and an… CONTINUE READING

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