Proteomics profiling of epidermal mucus secretion of a cichlid (Symphysodon aequifasciata) demonstrating parental care behavior

  title={Proteomics profiling of epidermal mucus secretion of a cichlid (Symphysodon aequifasciata) demonstrating parental care behavior},
  author={Kenny Chong and Shashikant B. Joshi and Lam Toong Jin and Alexander Chong Shu‐Chien},
The discus fish (Symphysodon aequifasciata) is a cichlid demonstrating advanced mode of parental care towards fry. Both male and female fish utilized epidermal mucus secreted from specialized epidermal cells to feed developing fry. We utilized proteomics to compare protein profile from parental and nonparental fish. Gel analysis revealed a total of 35 spots that were up‐regulated in parental mucus. In tandem, another 18 spots were uniquely expressed in parental mucus. MS analysis of these spots… 

Prolactin receptor mRNA is upregulated in discus fish (Symphysodon aequifasciata) skin during parental phase.

Skin mucus proteome map of European sea bass (Dicentrarchus labrax)

This is the first skin mucus proteome study and further transcriptional profiling of the identified proteins done on this bony fish species establishes a non‐invasive technique based on locating immune markers with a potential use for prevention and/or diagnosis of fish diseases.

Proteomics of Buccal Cavity Mucus in Female Tilapia Fish (Oreochromis spp.): A Comparison between Parental and Non-Parental Fish

Mouthbrooder oral mucus collectively showed increase levels of proteins related to cytoskeletal properties, glycolytic pathway and mediation of oxidative stress, suggesting cellular stress response, probably to support production of mucus during mouthbrooding phase.

Comparative proteome and peptidome analysis of the cephalic fluid secreted by Arapaima gigas (Teleostei: Osteoglossidae) during and outside parental care

Novel biochemical data is provided on the lateral line fluid which will enable future hypotheses-driven experiments to better understand the physiological roles of theateral line in chemical communication.

Skin mucus proteins of lumpsucker (Cyclopterus lumpus)

Dietary Yeast Cell Wall Extract Alters the Proteome of the Skin Mucous Barrier in Atlantic Salmon (Salmo salar): Increased Abundance and Expression of a Calreticulin-Like Protein

The calreticulin-like protein was identified as a possible biomarker for yeast-derived functional feeds since it showed the most consistent change in expression in both the mucus proteome and skin transcriptome and is expected to quicken the pace of research in the application of yeast cell wall extracts.



The male seahorse synthesizes and secretes a novel C‐type lectin into the brood pouch during early pregnancy

The aim of this project was to construct and characterize a cDNA library made from the tissue lining the pouch, in order to help understand the molecular mechanisms regulating its development and function, and it is shown that this protein is secreted in significant quantities into the pouch fluid specifically during early pregnancy.

Vitellogenin in the surface mucus of tilapia (Oreochromis mossambicus): Possibility for uptake by the free‐swimming embryos

The availability of VTGs in the mucus of the mother raises the possibility of continued nutrient supply from the mother for post-hatched embryonic tilapia, and alternatives of the yolk protein-like substances inside the gut of the embryos are considered.

Young of the Midas cichlid get biologically active nonnutrients by eating mucus from the surface of their parents

Fry (first mobile offspring) of the Midas cichlid,Cichlasoma citrinellum, feed on the epidermal mucus of their parents and grew faster initially than those raised without.

Differential display of skin mRNAs regulated under varying environmental conditions in a mudskipper

Northern blots demonstrate that mRNA levels of the actin-binding protein and the platelet-activating factor acetylhydrolase increased in the pectoral skins during seawater acclimation, suggesting that these four genes may be involved in the environmental adaptations.

Antimicrobial egg cleaning by the fringed darter (Perciformes: Percidae: Etheostoma crossopterum): implications of a novel component of parental care in fishes

  • J. KnouftL. PageM. Plewa
  • Biology, Environmental Science
    Proceedings of the Royal Society of London. Series B: Biological Sciences
  • 2003
Results from this study indicate that E. crossopterum males provide a novel form of vertebrate parental care, but also have implications regarding the evolution of parental care in fishes and transitional evolutionary stages from no parental care to male parental care.


Comparative proteomic profiling of murine skin.

Proteomic analysis of skin following heat or cold shock resulted in increased levels of HSP27, HSP60, and HSP70 suggesting involvement of these chaperones in the cutaneous response mechanism to temperature stress, and establishes a reference proteome map of BALB/c murine skin allowing the resolution of greater than 500 protein spots in a single two-dimensional polyacrylamide gel.