Proteomics out of the archive: Two-dimensional electrophoresis and mass spectrometry using HOPE-fixed, paraffin-embedded tissues.

@article{Khler2010ProteomicsOO,
  title={Proteomics out of the archive: Two-dimensional electrophoresis and mass spectrometry using HOPE-fixed, paraffin-embedded tissues.},
  author={Daniel K{\"a}hler and Christian Alexander and Holger Schultz and Mahdi Abdullah and Detlev Branscheid and Buko Lindner and Peter Zabel and Ekkehard Vollmer and Torsten Goldmann},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={2010},
  volume={58 3},
  pages={221-8}
}
Proteome analyses provide diagnostic information which can be essential for therapeutic predictions. The application of such techniques for analyzing paraffin-embedded tissue samples is widely hampered by the use of formalin fixation requiring antigen retrieval procedures in molecular pathology. In prior studies, the HEPES-glutamic acid buffer-mediated organic solvent protection effect (HOPE) technique of tissue fixation has been shown to provide a broad array of biochemical investigations with… CONTINUE READING