Proteomics of rat liver Golgi complex: minor proteins are identified through sequential fractionation.

@article{Taylor2000ProteomicsOR,
  title={Proteomics of rat liver Golgi complex: minor proteins are identified through sequential fractionation.},
  author={Rennae S Taylor and Christine C. Wu and Lara G Hays and Jimmy K. Eng and John R. Yates and Kathryn E. Howell},
  journal={Electrophoresis},
  year={2000},
  volume={21 16},
  pages={3441-59}
}
The discovery of novel proteins resident to the Golgi complex will fuel our future studies of Golgi structure/function and provide justification for proteomic analysis of this organelle. Our approach to Golgi proteomics was to first isolate and characterize the intact organelle free of proteins in transit by use of tissue pretreated with cycloheximide. Then the stacked Golgi fraction was fractionated into biochemically defined subfractions: Triton X-114 insoluble, aqueous, and detergent phases… CONTINUE READING

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