Proteomics of cypress pollen allergens using double and triple one-dimensional electrophoresis.

@article{Shahali2012ProteomicsOC,
  title={Proteomics of cypress pollen allergens using double and triple one-dimensional electrophoresis.},
  author={Youcef Shahali and Jean-Pierre Sutra and Iman Haddad and Jo{\"e}lle Vinh and Laurence Guilloux and Gabriel Peltre and H{\'e}l{\`e}ne S{\'e}n{\'e}chal and Pascal Poncet},
  journal={Electrophoresis},
  year={2012},
  volume={33 3},
  pages={462-9}
}
Italian cypress (Cupressus sempervirens, Cups) pollen causes allergic diseases in inhabitants of many of the cities surrounding the Mediterranean basin. However, allergens of Cups pollen are still poorly known. We introduce here a novel proteomic approach based on double one-dimensional gel electrophoresis (D1-DE) as an alternative to the 2-DE immunoblot, for the specific IgE screening of allergenic proteins from pollen extracts. The sequential one-dimensional combination of IEF and SDS-PAGE… CONTINUE READING