Proteomics of Primary Cilia by Proximity Labeling.

  title={Proteomics of Primary Cilia by Proximity Labeling.},
  author={David U. Mick and Rachel B Rodrigues and Ryan D. Leib and Christopher M Adams and Allis S. Chien and Steven P Gygi and Maxence V Nachury},
  journal={Developmental cell},
  volume={35 4},
While cilia are recognized as important signaling organelles, the extent of ciliary functions remains unknown because of difficulties in cataloguing proteins from mammalian primary cilia. We present a method that readily captures rapid snapshots of the ciliary proteome by selectively biotinylating ciliary proteins using a cilia-targeted proximity labeling enzyme (cilia-APEX). Besides identifying known ciliary proteins, cilia-APEX uncovered several ciliary signaling molecules. The kinases PKA… CONTINUE READING
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