Proteomics and phosphoproteomics analysis of human lens fiber cell membranes.

  title={Proteomics and phosphoproteomics analysis of human lens fiber cell membranes.},
  author={Zhen Wang and Jun Han and Larry David and Kevin L. Schey},
  journal={Investigative ophthalmology \& visual science},
  volume={54 2},
PURPOSE The human lens fiber cell insoluble membrane fraction contains important membrane proteins, cytoskeletal proteins, and cytosolic proteins that are strongly associated with the membrane. The purpose of this study was to characterize the lens fiber cell membrane proteome and phosphoproteome from human lenses. METHODS HPLC-mass spectrometry-based multidimensional protein identification technology (MudPIT), without or with phosphopeptide enrichment, was applied to study the proteome and… 

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