Proteomic identification of 14-3-3zeta as a mitogen-activated protein kinase-activated protein kinase 2 substrate: role in dimer formation and ligand binding.

@article{Powell2003ProteomicIO,
  title={Proteomic identification of 14-3-3zeta as a mitogen-activated protein kinase-activated protein kinase 2 substrate: role in dimer formation and ligand binding.},
  author={David W. Powell and Madhavi J. Rane and Brian A Joughin and Ralitsa I. Kalmukova and Jeong-Ho Hong and Bruce Tidor and W. Dean and William M. Pierce and Jon B. Klein and Michael B. Yaffe and Kenneth R Mcleish},
  journal={Molecular and cellular biology},
  year={2003},
  volume={23 15},
  pages={5376-87}
}
Mitogen-activated protein kinase (MAPK)-activated protein kinase 2 (MAPKAPK2) mediates multiple p38 MAPK-dependent inflammatory responses. To define the signal transduction pathways activated by MAPKAPK2, we identified potential MAPKAPK2 substrates by using a functional proteomic approach consisting of in vitro phosphorylation of neutrophil lysate by active recombinant MAPKAPK2, protein separation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and phosphoprotein… CONTINUE READING

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