Proteomic comparison of two fractions derived from the transsynaptic scaffold.

  title={Proteomic comparison of two fractions derived from the transsynaptic scaffold.},
  author={Greg R Phillips and Laurence Florens and Hidekazu Tanaka and Zin Z Khaing and Lazar Fidler and John R. Yates and David R. Colman},
  journal={Journal of neuroscience research},
  volume={81 6},
A fraction derived from presynaptic specializations (presynaptic particle fraction; PPF) can be separated from postsynaptic densities (PSD) by adjusting the pH of Triton X-100 (TX-100) extraction of isolated transsynaptic scaffolds. Solubilization of the PPF corresponds to disruption of the presynaptic specialization. We show that the PPF is insoluble to repeated TX-100 extraction at pH 6.0 but becomes soluble in detergent at pH 8.0. By immunolocalization, we find that the major proteins of the… CONTINUE READING

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