Proteomic characterization of harvested pseudopodia with differential gel electrophoresis and specific antibodies

@article{Beckner2005ProteomicCO,
  title={Proteomic characterization of harvested pseudopodia with differential gel electrophoresis and specific antibodies},
  author={Marie E. Beckner and Xuan Chen and Jiyan An and Billy W. Day and Ian F Pollack},
  journal={Laboratory Investigation},
  year={2005},
  volume={85},
  pages={316-327}
}
Malignant gliomas (astrocytomas) are lethal tumors that invade the brain. Invasive cell migration is initiated by extension of pseudopodia into interstitial spaces. In this study, U87 glioma cells formed pseudopodia in vitro as cells pushed through 3 μm pores of polycarbonate membranes. Harvesting pseudopodia in a novel two-step method provided material for proteomic analysis. Differences in the protein profiles of pseudopodia and whole cells were found using differential gel electrophoresis… CONTINUE READING