Proteomic and phosphoproteomic analysis of Picea wilsonii pollen development under nutrient limitation.

@article{Chen2012ProteomicAP,
  title={Proteomic and phosphoproteomic analysis of Picea wilsonii pollen development under nutrient limitation.},
  author={Yanmei Chen and Peng Liu and Wolfgang Hoehenwarter and Jinxing Lin},
  journal={Journal of proteome research},
  year={2012},
  volume={11 8},
  pages={4180-90}
}
The pollen tube is a tip-growing system that delivers sperm to the ovule and thus is essential for sexual plant reproduction. Sucrose and other microelements act as nutrients and signaling molecules through pathways that are not yet fully understood. Taking advantage of high-throughput liquid chromatography coupled to mass spectrometry (LC-MS), we performed a label-free shotgun proteomic analysis of pollen in response to nutrient limitation using mass accuracy precursor alignment. We compared… CONTINUE READING
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