Proteomic analysis of the adaptive response of rat renal proximal tubules to metabolic acidosis.

@article{Curthoys2007ProteomicAO,
  title={Proteomic analysis of the adaptive response of rat renal proximal tubules to metabolic acidosis.},
  author={Norman P. Curthoys and Lynn Taylor and Jason D. Hoffert and Mark A. Knepper},
  journal={American journal of physiology. Renal physiology},
  year={2007},
  volume={292 1},
  pages={F140-7}
}
Proximal tubules were isolated from control and acidotic rats by collagenase digestion and Percoll density gradient centrifugation. Western blot analysis indicated that the tubules were approximately 95% pure. The samples were analyzed by two-dimensional difference gel electrophoresis (DIGE) and DeCyder software was used to quantify the temporal changes in proteins that exhibit enhanced or reduced expression. The mass-to-charge ratios and the amino acid sequences of the recovered tryptic… CONTINUE READING