Proteomic analysis of synaptosomal protein expression reveals that cerebral ischemia alters lysosomal Psap processing.

@article{Costain2010ProteomicAO,
  title={Proteomic analysis of synaptosomal protein expression reveals that cerebral ischemia alters lysosomal Psap processing.},
  author={Willard J. Costain and Arsalan S. Haqqani and Ingrid Rasquinha and M Giguere and Jacqueline Slinn and Bogdan Zurakowski and Danica B. Stanimirovi{\'c}},
  journal={Proteomics},
  year={2010},
  volume={10 18},
  pages={3272-91}
}
Cerebral ischemia (CI) induces dramatic changes in synaptic structure and function that precedes delayed post-ischemic neuronal death. Here, a proteomic analysis was used to identify the effects of focal CI on synaptosomal protein levels. Contralateral and ipsilateral synaptosomes, prepared from adult mice subjected to 60 min middle cerebral artery occlusion, were isolated following 3, 6 and 20 h of reperfusion. Synaptosomal protein samples (n=3) were labeled using the cleavable ICAT system… CONTINUE READING