Proteomic analysis of soybean nodule cytosol.

  title={Proteomic analysis of soybean nodule cytosol.},
  author={Nathan W. Oehrle and Annamraju D. Sarma and James K. Waters and David W. Emerich},
  volume={69 13},
An isolation procedure for soybean (Glycine max L. cv Williams 82) nodule cytosol proteins was developed which greatly improved protein resolution by two-dimensional polyacrylamide gel electrophoresis. The most abundant proteins were selected and analyzed by mass spectrometry. The identified proteins were categorized by function (% of total proteins analyzed): carbon metabolism (28%), nitrogen metabolism (12%), reactive oxygen metabolism (12%) and vesicular trafficking (11%). The first three… CONTINUE READING
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