Proteomic analysis of human O6-methylguanine-DNA methyltransferase by affinity chromatography and tandem mass spectrometry.

@article{Niture2005ProteomicAO,
  title={Proteomic analysis of human O6-methylguanine-DNA methyltransferase by affinity chromatography and tandem mass spectrometry.},
  author={Suryakant K. Niture and Catalin E. Doneanu and Chinavenmeni S. Velu and Nathan I. Bailey and Kalkunte Srirangachar Srivenugopal},
  journal={Biochemical and biophysical research communications},
  year={2005},
  volume={337 4},
  pages={
          1176-84
        }
}
Recent evidence suggests that human O(6)-methylguanine-DNA methyltransferase (MGMT), a DNA repair protein that protects the genome against mutagens and accords tumor resistance to many anticancer alkylating agents, may have other roles besides repair. Therefore, we isolated MGMT-interacting proteins from extracts of HT29 human colon cancer cells using affinity chromatography on MGMT-Sepharose. Specific proteins bound to this column were identified by electrospray ionization tandem mass… CONTINUE READING
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