Proteomic analysis of exosomes isolated from human malignant pleural effusions.

@article{Bard2004ProteomicAO,
  title={Proteomic analysis of exosomes isolated from human malignant pleural effusions.},
  author={Martin P. L. Bard and Joost P.J.J. Hegmans and Annabrita Hemmes and Theo M. Luider and Rob Willemsen and L A Severijnen and Jan P. van Meerbeeck and Sjaak A. Burgers and Henk C. Hoogsteden and Bart N Lambrecht},
  journal={American journal of respiratory cell and molecular biology},
  year={2004},
  volume={31 1},
  pages={
          114-21
        }
}
Exosomes are membrane vesicles from endosomal origin secreted by various cells such as hematopoietic, epithelial, and tumor cells. Exosomes secreted by tumor cells contain specific antigens potentially useful for immunotherapeutic purposes. Our aim was to determine if exosomes are present in human cancerous pleural effusions and to identify their proteomic content. Exosomes were purified by sucrose gradient ultracentrifugation, and electron microscopy was used to check both concentration and… CONTINUE READING
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