Proteomic analysis of cell lines to identify the irinotecan resistance proteins

  title={Proteomic analysis of cell lines to identify the irinotecan resistance proteins},
  author={Xing-Chen Peng and Feng-ming Gong and Meng Wei and X. Chen and Ye Chen and Ke Cheng and F. Gao and Feng Xu and Feng Bi and Jiyan Liu},
  journal={Journal of Biosciences},
Chemotherapeutic drug resistance is a frequent cause of treatment failure in colon cancer patients. Several mechanisms have been implicated in drug resistance. However, they are not sufficient to exhaustively account for this resistance emergence. In this study, two-dimensional gel electrophoresis (2-DE) and the PDQuest software analysis were applied to compare the differential expression of irinotecan-resistance-associated protein in human colon adenocarcinoma LoVo cells and irinotecan… 

RhoA regulates resistance to irinotecan by regulating membrane transporter and apoptosis signaling in colorectal cancer

In conclusion, it is suggested that, in addition to survival, proliferation, migration, adhesion, cell cycle and gene transcription, RhoA is also involved in chemoresistance by regulating the expression of membrane transporter and apoptosis protein in colorectal cancer.

Inhibition of the Multidrug Resistance P-Glycoprotein: Time for a Change of Strategy?

This review provides a description of several alternative approaches to overcome the activity of P-gp in drug-resistant cells, which include drugs that specifically target resistant cells, novel nanotechnologies to provide high-dose, targeted delivery of anticancer drugs, and approaches to reduce the expression ofP-gp within tumors.

Aldo-Keto Reductases and Cancer Drug Resistance

Inhibitors of the NRF2 system or pan-AKR1C inhibitors offer promise to surmount cancer drug resistance and/or synergize the effects of existing drugs.

Irinotecan—Still an Important Player in Cancer Chemotherapy: A Comprehensive Overview

A comprehensive overview on irinotecan’s molecular mode of action, metabolism, pharmacogenetics, and toxicity is given and crucial mechanisms of tumor cells’ resistance to the active metabolite, ethyl-10-hydroxy-camptothecin (SN-38) are outlined.

Preventive Cancer Stem Cell‐Based Vaccination Reduces Liver Metastasis Development in a Rat Colon Carcinoma Syngeneic Model

It is demonstrated that a CSC‐based vaccine reduces efficiently both tumor volume and occurrence in a rat colon carcinoma syngeneic model, the first work analyzing the potential of a C SCs‐based vaccination to prevent liver metastasis development.

Regulation of Aldo–Keto Reductases in Human Diseases

This review summarizes the current knowledge on AKR regulation by transcription factors and other mediators in human diseases and identifies many transcription factors have been identified to regulate the expression of human AKR genes.

Novel pharmacogenomic markers of irinotecan-induced severe toxicity in metastatic colorectal cancer patients

It is suggested that children under the age of five should be supervised by an adult rather than rely on their parents for most of the day-to-day activities.


  • Computer Science
  • 2021
A novel deep learning approach to feature selection that addresses both challenges simultaneously, pre-train the network using unlabeled samples within a self-supervised learning framework and fine-tune the pre-trained network to discover relevant features using labeled samples.


A novel deep learning approach to feature selection that addresses both challenges simultaneously and discovers relevant features that provide superior prediction performance compared to the state-of-the-art benchmarks in practical scenarios where there is often limited labeled data and high correlations among features.



Proteomics for studying cancer cells and the development of chemoresistance

The possibilities in studying cancer biology and development of chemoresistance in cancer treatment using the proteomic approach are reviewed.

Proteomic analysis of liver cancer cells treated with suberonylanilide hydroxamic acid

Using proteomics approaches, a variety of differentially expressed proteins were identified in HepG2 cancer cells before and after treatment with SAHA, and a better understanding of the molecular mechanisms underlying SAHA-mediated antitumor effects at the protein level is enabled.

ABC-transporters: implications on drug resistance from microorganisms to human cancers.

  • H. Lage
  • Biology, Medicine
    International journal of antimicrobial agents
  • 2003

Multidrug resistance: molecular mechanisms and clinical relevance

  • V. Ling
  • Biology, Medicine
    Cancer Chemotherapy and Pharmacology
  • 1997
It would be interesting to determine whether patients who fail treatment in the presence of chemosensitizing agents acquire other MDR mechanisms, and to determine which ABC transporters are clinically relevant.

Increased expression of epidermal fatty acid binding protein, cofilin, and 14‐3‐3‐σ (stratifin) detected by two‐dimensional gel electrophoresis, mass spectrometry and microsequencing of drug‐resistant human adenocarcinoma of the pancreas

Three proteins, E‐FABP, cofilin, and 14-3‐3‐σ (stratifin) was found to be overexpressed only in the mitoxantrone‐selected atypical multidrug‐resistant cell line, and the possible significance of these findings is discussed.

The role of oncogenes in drug resistance

It is hoped that better undertsnading on the role of oncogenes in drug resistance will invoke ideas on new approaches to enhance the cytotoxicity of the standard chemotherapeutic agents by functional perturbation of resistance-inducing oncogens.

Transport of amphipathic anions by human multidrug resistance protein 3.

MRP3, like MRP1 and cMOAT, is concluded to be competent in the transport of glutathione S-conjugates, glucuronides, and methotrexate, albeit at low to moderate affinity.

Frequent methylation-associated silencing of a candidate tumor-suppressor, CRABP1, in esophageal squamous-cell carcinoma

Results indicate that CRABP1 appears to have a tumor-suppressor function in esophageal epithelium, and its epigenetic silencing may play a pivotal role during esophagal carcinogenesis.