Proteomic analysis of cell lines to identify the irinotecan resistance proteins

  title={Proteomic analysis of cell lines to identify the irinotecan resistance proteins},
  author={Xing-chen Peng and Feng-ming Gong and Meng Wei and X. Chen and Ye Chen and Ke Cheng and F. Gao and Feng Xu and Feng Bi and Jiyan Liu},
  journal={Journal of Biosciences},
Chemotherapeutic drug resistance is a frequent cause of treatment failure in colon cancer patients. Several mechanisms have been implicated in drug resistance. However, they are not sufficient to exhaustively account for this resistance emergence. In this study, two-dimensional gel electrophoresis (2-DE) and the PDQuest software analysis were applied to compare the differential expression of irinotecan-resistance-associated protein in human colon adenocarcinoma LoVo cells and irinotecan… 
RhoA regulates resistance to irinotecan by regulating membrane transporter and apoptosis signaling in colorectal cancer
In conclusion, it is suggested that, in addition to survival, proliferation, migration, adhesion, cell cycle and gene transcription, RhoA is also involved in chemoresistance by regulating the expression of membrane transporter and apoptosis protein in colorectal cancer.
Inhibition of the Multidrug Resistance P-Glycoprotein: Time for a Change of Strategy?
This review provides a description of several alternative approaches to overcome the activity of P-gp in drug-resistant cells, which include drugs that specifically target resistant cells, novel nanotechnologies to provide high-dose, targeted delivery of anticancer drugs, and approaches to reduce the expression ofP-gp within tumors.
Aldo-Keto Reductases and Cancer Drug Resistance
Inhibitors of the NRF2 system or pan-AKR1C inhibitors offer promise to surmount cancer drug resistance and/or synergize the effects of existing drugs.
Irinotecan—Still an Important Player in Cancer Chemotherapy: A Comprehensive Overview
A comprehensive overview on irinotecan’s molecular mode of action, metabolism, pharmacogenetics, and toxicity is given and crucial mechanisms of tumor cells’ resistance to the active metabolite, ethyl-10-hydroxy-camptothecin (SN-38) are outlined.
Preventive Cancer Stem Cell‐Based Vaccination Reduces Liver Metastasis Development in a Rat Colon Carcinoma Syngeneic Model
It is demonstrated that a CSC‐based vaccine reduces efficiently both tumor volume and occurrence in a rat colon carcinoma syngeneic model, the first work analyzing the potential of a C SCs‐based vaccination to prevent liver metastasis development.
Regulation of Aldo–Keto Reductases in Human Diseases
This review summarizes the current knowledge on AKR regulation by transcription factors and other mediators in human diseases and identifies many transcription factors have been identified to regulate the expression of human AKR genes.
Novel pharmacogenomic markers of irinotecan-induced severe toxicity in metastatic colorectal cancer patients
It is suggested that children under the age of five should be supervised by an adult rather than rely on their parents for most of the day-to-day activities.


Proteomics for studying cancer cells and the development of chemoresistance
The possibilities in studying cancer biology and development of chemoresistance in cancer treatment using the proteomic approach are reviewed.
Proteomic analysis of liver cancer cells treated with suberonylanilide hydroxamic acid
Using proteomics approaches, a variety of differentially expressed proteins were identified in HepG2 cancer cells before and after treatment with SAHA, and a better understanding of the molecular mechanisms underlying SAHA-mediated antitumor effects at the protein level is enabled.
Cellular and molecular determinants of cisplatin resistance.
  • R. Perez
  • Biology, Medicine
    European journal of cancer
  • 1998
O Ongoing biochemical modulation and translational correlative trials should clarify which specific mechanisms are most relevant to clinical cisplatin resistance and should identify potential targets for pharmacological or molecular intervention.
ABC-transporters: implications on drug resistance from microorganisms to human cancers.
  • H. Lage
  • Biology, Medicine
    International journal of antimicrobial agents
  • 2003
The current state of knowledge of the functional and structural similarities among ABC-transporters in prokaryotic and eukaryotic cells and their impact on multidrug resistance are discussed.
Increased resistance of tumor cells to daunorubicin after transfection of cDNAs coding for anthracycline inactivating enzymes.
Coadministration of DRC reductase inhibitors in DRC chemotherapy may be desirable since this would reduce the formation of the cardiotoxic alcohol metabolite and prevent drug resistance.
Multidrug resistance: molecular mechanisms and clinical relevance
  • V. Ling
  • Biology, Medicine
    Cancer Chemotherapy and Pharmacology
  • 1997
It would be interesting to determine whether patients who fail treatment in the presence of chemosensitizing agents acquire other MDR mechanisms, and to determine which ABC transporters are clinically relevant.
Increased expression of epidermal fatty acid binding protein, cofilin, and 14‐3‐3‐σ (stratifin) detected by two‐dimensional gel electrophoresis, mass spectrometry and microsequencing of drug‐resistant human adenocarcinoma of the pancreas
Three proteins, E‐FABP, cofilin, and 14-3‐3‐σ (stratifin) was found to be overexpressed only in the mitoxantrone‐selected atypical multidrug‐resistant cell line, and the possible significance of these findings is discussed.
The role of oncogenes in drug resistance
  • Dihua Yu
  • Biology, Medicine
  • 2004
It is hoped that better undertsnading on the role of oncogenes in drug resistance will invoke ideas on new approaches to enhance the cytotoxicity of the standard chemotherapeutic agents by functional perturbation of resistance-inducing oncogens.
Anthracyclines and their C-13 alcohol metabolites: growth inhibition and DNA damage following incubation with human tumor cells in culture
These studies in human leukemia and human glioblastoma cell lines support the hypothesis that idarubicinol plays an important role in the antitumor activity of idarUBicin and that the activities of idracycline and idarubsinol are related to their ability to damage DNA.
Transport of amphipathic anions by human multidrug resistance protein 3.
MRP3, like MRP1 and cMOAT, is concluded to be competent in the transport of glutathione S-conjugates, glucuronides, and methotrexate, albeit at low to moderate affinity.