Proteomic analysis for process development and control of therapeutic protein separation from human plasma.

@article{Yang2009ProteomicAF,
  title={Proteomic analysis for process development and control of therapeutic protein separation from human plasma.},
  author={Xinli Yang and James G Clifton and Feilei Huang and Spomenka Kova{\vc} and Douglas C. Hixson and Djuro Josi{\'c}},
  journal={Electrophoresis},
  year={2009},
  volume={30 7},
  pages={1185-93}
}
The use of proteomics technology during the development of a new process for plasma protein separation was demonstrated. In a two-step process, the two most abundant proteins, HSA and IgG, were removed in a first step of anion-exchange chromatography using a gel with very high capacity. Subsequently, two fractions containing medium and low abundance proteins were re-chromatographed on a smaller column with the same type of gel. Collected fractions were separated by SDS-PAGE and 2-D… CONTINUE READING