Proteomic analysis demonstrates activator- and chromatin-specific recruitment to promoters.

@article{Sikorski2012ProteomicAD,
  title={Proteomic analysis demonstrates activator- and chromatin-specific recruitment to promoters.},
  author={Timothy W Sikorski and Yoo Jin Joo and Scott B. Ficarro and Manor Askenazi and Stephen Buratowski and Jarrod A. Marto},
  journal={The Journal of biological chemistry},
  year={2012},
  volume={287 42},
  pages={35397-408}
}
In-depth characterization of RNA polymerase II preinitiation complexes remains an important and challenging goal. We used quantitative mass spectrometry to explore context-dependent Saccharomyces cerevisiae preinitiation complex formation at the HIS4 promoter reconstituted on naked and chromatinized DNA templates. The transcription activators Gal4-VP16 and Gal4-Gcn4 recruited a limited set of chromatin-related coactivator complexes, namely the chromatin remodeler Swi/Snf and histone… CONTINUE READING