Proteome-wide analysis of protein carboxy termini: C terminomics

@article{Schilling2010ProteomewideAO,
  title={Proteome-wide analysis of protein carboxy termini: C terminomics},
  author={Oliver Schilling and O. De La Barre and Pitter F. Huesgen and Christopher M. Overall},
  journal={Nature Methods},
  year={2010},
  volume={7},
  pages={508-511}
}
As proteome-wide C-terminal sequence analysis has been largely intractable, we developed a polymer-based enrichment approach to profile protein C-terminal peptides by mass spectrometry and identified hundreds of C-terminal peptides in the Escherichia coli proteome. We isotopically labeled GluC protease–digested and undigested samples and identified GluC substrates and their cleavage sites by quantification of neo–C-terminal peptides. Our method thus enables global annotation of protein C… CONTINUE READING
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