Proteome analysis of silk gland proteins from the silkworm, Bombyx mori.

  title={Proteome analysis of silk gland proteins from the silkworm, Bombyx mori.},
  author={Pingbo Zhang and Yoichi Aso and Kohji Yamamoto and Yutaka Banno and Yongqiang Wang and Kozo Tsuchida and Yutaka Kawaguchi and Hiroshi Fujii},
  volume={6 8},
The silk gland of Bombyx mori is an organ specialized for the synthesis and secretion of silk proteins. We report here the resolution of silk gland proteins by 2-DE and the identification of many of those proteins. This was accomplished by dissecting the glands into several sections, with each exhibiting more than 400 protein spots by 2-DE, of which 100 spots were excised and characterized by in-gel digestion followed by PMF. Ninety-three proteins were tentatively identified. These were then… CONTINUE READING

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