Proteome analysis of recombinant xylose‐fermenting Saccharomyces cerevisiae

@article{Salusjrvi2003ProteomeAO,
  title={Proteome analysis of recombinant xylose‐fermenting Saccharomyces cerevisiae},
  author={Laura Salusj{\"a}rvi and Marjo Anneli Poutanen and Juha-Pekka Pitk{\"a}nen and Heini Koivistoinen and Aristos A. Aristidou and Nisse Kalkkinen and Laura Ruohonen and Merja Penttil{\"a}},
  journal={Yeast},
  year={2003},
  volume={20}
}
Introduction of an active xylose utilization pathway into Saccharomyces cerevisiae, which does not naturally ferment pentose sugars, is likely to have a major impact on the overall cellular metabolism as the carbon introduced to the cells will now flow through the pentose phosphate pathway. The metabolic responses in the recombinant xylose‐fermenting S. cerevisiae were studied at the proteome level by comparative two‐dimensional gel electrophoresis of cellular proteins within a pH range of 3–10… 
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Metabolic flux analysis of xylose metabolism in recombinant Saccharomyces cerevisiae using continuous culture.
Regulation of xylose metabolism in recombinant Saccharomyces cerevisiae
TLDR
The results indicate that the metabolism of yeast growing on xylose corresponds neither to that of fully glucose repressed cells nor that of derepressed cells, which may be one of the major reasons for the suboptimal fermentation ofxylose by recombinant S. cerevisiae strains.
Molecular Basis for Anaerobic Growth of Saccharomyces cerevisiae on Xylose, Investigated by Global Gene Expression and Metabolic Flux Analysis
TLDR
It is concluded that anaerobic growth of the yeast on xylose is ultimately limited by the rate of ATP production and not by the redox balance per se, although theredox imbalance, in turn, limits ATP production.
Transcription analysis of recombinant Saccharomyces cerevisiae reveals novel responses to xylose
TLDR
It is suggested that cells metabolizing xylose are not in a completely repressed or in a derepressed state either, indicating thatxylose was recognized neither as a fermentable nor as a respirative carbon source.
Limitations in Xylose-Fermenting Saccharomyces cerevisiae, Made Evident through Comprehensive Metabolite Profiling and Thermodynamic Analysis
TLDR
Thermodynamic analysis complemented by enzyme kinetic considerations suggested that activities of pentose phosphate pathway enzymes and the pool of fructose-6-phosphate are potential factors limiting xylose utilization.
Proteome analysis of the xylose‐fermenting mutant yeast strain TMB 3400
TLDR
The results of the proteome analysis were in good agreement with a parallel study in which rationally designed overexpression of XR, XDH and the non‐oxidative pentose phosphate pathway resulted in similar improvement in xylose utilization, which demonstrates the usefulness of proteomeAnalysis for the identification of target genes for further metabolic engineering strategies in industrial yeast strains.
Transcription analysis of recombinant industrial and laboratory Saccharomyces cerevisiae strains reveals the molecular basis for fermentation of glucose and xylose
TLDR
Xylose-utilizing S. cerevisiae strains may recognize xylose as a non-fermentable carbon source, which induces a starvation response and adaptation to oxidative stress, resulting in the increased expression of stress-response genes.
Effect of glucose on xylose utilization in Saccharomyces cerevisiae harboring the xylose reductase gene
TLDR
The results of this study suggest that xylose utilization would be improved by activation of hexose transporters induced by glucose (rather thanxylose) reductase expression.
Xylose chemostat isolates of Saccharomyces cerevisiae show altered metabolite and enzyme levels compared with xylose, glucose, and ethanol metabolism of the original strain
TLDR
This study focused on the recovery and characterization of xylose chemostat isolates of a S. cerevisiae strain that overexpresses xylOSE reductase- and xylitol dehydrogenase-encoding genes from Pichia stipitis and the gene encoding the endogenous xylulokinase.
Comparative Proteome Analysis of Saccharomyces cerevisiae Grown in Chemostat Cultures Limited for Glucose or Ethanol*
TLDR
It is shown here that the combined approach of chemostat cultivation and comprehensive proteome analysis allowed us to study the primary effect of single limiting conditions on the yeast proteome and unravel which processes in the central carbon metabolism were regulated at the level of the proteome, and which processes at thelevel of transcriptome.
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References

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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
Xylose transport, xylose reductase, and xylitol dehydrogenase activities are demonstrated in Saccharomyces cerevisiae, and in vivo conversion of 14C‐xylose in S. Cerevisiae is demonstrated andxylitol is detected, although no significant levels of any other 14C-labeled metabolites (e. g., ethanol) are observed.
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