Proteome analysis of brain proteins in Alzheimer's disease: subproteomics following sequentially extracted protein preparation.

@article{Shiozaki2004ProteomeAO,
  title={Proteome analysis of brain proteins in Alzheimer's disease: subproteomics following sequentially extracted protein preparation.},
  author={Aiko Shiozaki and Teruyuki Tsuji and Ryuichi Kohno and Jun Kawamata and Kengo Uemura and Hiroshi Teraoka and Shun Shimohama},
  journal={Journal of Alzheimer's disease : JAD},
  year={2004},
  volume={6 3},
  pages={257-68}
}
Quantitative proteome analysis of Alzheimer's disease (AD) brains was performed using two-dimensional (2-D) gels in order to find out the pathological protein expression in AD. We sequentially extracted brain proteins using two distinct sample solutions, yielding different protein fractions (fraction A and B). These fractions showed distinct 2-DE patterns with high resolution and excellent reproducibility. In fraction A (solubilized by urea and Nonidet P-40 (NP-40)), approximately 1300 protein… CONTINUE READING

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