Proteome analysis of an aerobic hyperthermophilic crenarchaeon, Aeropyrum pernix K1.

  title={Proteome analysis of an aerobic hyperthermophilic crenarchaeon, Aeropyrum pernix K1.},
  author={Syuji Yamazaki and Jun Yamazaki and Keiko Nishijima and Rie Otsuka and Miyako Mise and Hanako Ishikawa and Kazumi Sasaki and Shin-ichi Tago and Katsumi Isono},
  journal={Molecular & cellular proteomics : MCP},
  volume={5 5},
We analyzed the proteome of a crenararchaeon, Aeropyrum pernix K1, by using the following four methods: (i) two-dimensional PAGE followed by MALDI-TOF MS, (ii) one-dimensional SDS-PAGE in combination with two-dimensional LC-MS/MS, (iii) multidimensional LC-MS/MS, and (iv) two-dimensional PAGE followed by amino-terminal amino acid sequencing. These methods were found to be complementary to each other, and biases in the data obtained in one method could largely be compensated by the data obtained… CONTINUE READING


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