Proteome analysis of Saccharomyces cerevisiae: a methodological outline.

@article{Fey1997ProteomeAO,
  title={Proteome analysis of Saccharomyces cerevisiae: a methodological outline.},
  author={Stephen John Fey and Arthur Nawrocki and Martin R Larsen and Angelika G{\"o}rg and Peter Roepstorff and Georgina Skews and Rosie Williams and Peter Mose Larsen},
  journal={Electrophoresis},
  year={1997},
  volume={18 8},
  pages={1361-72}
}
Proteome analysis offers a unique means of identifying important proteins, characterizing their modifications and beginning to describe their function. This is achieved through the combination of two technologies: protein separation and selection by two-dimensional gel electrophoresis, and protein identification and characterization by mass spectrometry. This methodological outline sketches the strengths and weaknesses of the two central technologies used, and provides both practical tips and… CONTINUE READING