Proteome analysis of Rickettsia conorii by two-dimensional gel electrophoresis coupled with mass spectrometry.

@article{Renesto2005ProteomeAO,
  title={Proteome analysis of Rickettsia conorii by two-dimensional gel electrophoresis coupled with mass spectrometry.},
  author={Patricia Renesto and Sa{\"i}d Azza and Alain R. Dolla and Patrick Fourquet and Guy Vestris and Jean-Pierre E. Gorvel and Didier Raoult},
  journal={FEMS microbiology letters},
  year={2005},
  volume={245 2},
  pages={231-8}
}
The availability of genome sequence offers the opportunity to further expand our knowledge about proteins expressed by Rickettsia conorii, strictly intracellular bacterium responsible for Mediterranean spotted fever. Using two-dimensional polyacrylamide gel electrophoresis combined with MALDI-TOF mass spectrometry, we established the first reference map of R. conorii proteome. This approach also allowed identification of GroEL as the major antigen recognized by rabbit serum and sera of infected… CONTINUE READING
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