Proteome analysis of Cry4Ba toxin-interacting Aedes aegypti lipid rafts using geLC-MS/MS.

@article{Bayyareddy2012ProteomeAO,
  title={Proteome analysis of Cry4Ba toxin-interacting Aedes aegypti lipid rafts using geLC-MS/MS.},
  author={Krishnareddy Bayyareddy and Xiang Zhu and Ronald Orlando and Michael J Adang},
  journal={Journal of proteome research},
  year={2012},
  volume={11 12},
  pages={5843-55}
}
Lipid rafts are microdomains in the plasma membrane of eukaryotic cells. Among their many functions, lipid rafts are involved in cell toxicity caused by pore forming bacterial toxins including Bacillus thuringiensis (Bt) Cry toxins. We isolated lipid rafts from brush border membrane vesicles (BBMV) of Aedes aegypti larvae as a detergent resistant membrane (DRM) fraction on density gradients. Cholesterol, aminopeptidase (APN), alkaline phosphatase (ALP) and the raft marker flotillin were… CONTINUE READING
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