Proteome analysis for the identification of in vivo estrogen‐regulated proteins in bone

@article{Pastorelli2005ProteomeAF,
  title={Proteome analysis for the identification of in vivo estrogen‐regulated proteins in bone},
  author={R. Pastorelli and Donatella Carpi and L. Airoldi and C. Chiabrando and R. Bagnati and R. Fanelli and S. Mov{\'e}rare and C. Ohlsson},
  journal={PROTEOMICS},
  year={2005},
  volume={5}
}
Estrogen deficiency results in a reduced bone mass, which can be prevented by treatment with estrogens. This study used a proteomic approach for the first time to obtain a global perspective of estrogens' effects on whole‐bone proteins. Bone proteome profiles were examined in three groups of mice: (1) sham‐operated with normal ovarian functions, (2) ovariectomised and (3) ovariectomised with estrogen replacement therapy. Bone proteins extracted from the humerus were separated by 2‐DE and… Expand
Rapid label-free identification of estrogen-induced differential protein expression in vivo from mouse brain and uterine tissue.
TLDR
A very significant decrease in the abundance of glutathione S-transferase recognized as a marker of estrogen's impact was confirmed and revealed potential new protein markers such as desmin and lumican that are critical components of cytoskeletal arrangement and, hence, regulation of their abundance could contribute to major morphological changes in the uterus occurring upon estrogenic stimulation. Expand
Method development of efficient protein extraction in bone tissue for proteome analysis.
TLDR
It was demonstrated that the developed extraction method of proteins in bone tissue was very efficient for comprehensive analysis of bone proteome and might be helpful for clarifying the mechanisms of bone diseases. Expand
Differential expression of both extracellular and intracellular proteins is involved in the lethal or nonlethal phenotypic variation of BrtlIV, a murine model for osteogenesis imperfecta
TLDR
Differentially expressed proteins identified in this model offer a starting point for elucidating the molecular basis of phenotypic variability, a characteristic common to many genetic disorders. Expand
Heat shock protein 60 causes osteoclastic bone resorption via toll-like receptor-2 in estrogen deficiency.
TLDR
HSP60 and TLR-2 are novel mediators of estrogen-deficiency-induced bone loss by stimulating the production and secretion of HSP60 from OCs lineages. Expand
The Regulation of MS-KIF18A Expression and Cross Talk with Estrogen Receptor
TLDR
It is demonstrated that ERα is a defined cargo of MS-KIF18A and added novel insight on the role of estrogen in regulation ofMS-Kif18A expression both in vivo and in vitro. Expand
Effects of cigarette smoking on the human urinary proteome.
TLDR
The data show for the first time that smoking can affect the expression profile of urinary proteins and changes in abundance of pancreatic alpha amylase and zinc-alpha-2-glycoprotein, which after proper validation, might be candidate biomarkers of diseases resulting from exposure to tobacco smoke. Expand
Proteomic and Transcriptomic Approaches for Studying Bone Regeneration in Health and Systemically Compromised Conditions
TLDR
It is suggested that future research will leverage the synergy of omics with statistical modeling and bioinformatics to prompt the understanding of the biology underpinning bone formation in health and medically compromised conditions. Expand
Comparative serum proteome expression of the steroid-induced femoral head osteonecrosis in adults
TLDR
Significantly lower levels of complement component 3 (C3), C3, C4, inter-α-trypsin inhibitor heavy chain H4 and α-2-macroglobulin were found in the serum of patients with SONFH, indicating that multiple pathological reactions occur in S onFH and these proteins may serve as potential biomarkers for the diagnosis of SonFH. Expand
Comparative serum proteome expression of osteonecrosis of the femoral head in adults.
TLDR
High--throughput serum proteomic findings indicated that multiple pathological reactions presumably occurred in ONFH, which was associated with various serum protein expressions. Expand
Proteomic analysis of cartilage‐ and bone‐associated samples
TLDR
The future prospects of applying proteomic techniques to investigate key events, such as cellular signal transduction, in cartilage‐ and bone‐derived cells can immediately benefit from the present knowledge. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 42 REFERENCES
Identification of Estrogen‐Regulated Genes of Potential Importance for the Regulation of Trabecular Bone Mineral Density
  • M. Lindberg, S. Movérare, +5 authors C. Ohlsson
  • Biology, Medicine
  • Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
  • 2002
TLDR
Four previously known and three novel estrogen‐regulated genes of potential importance for the trabecular bone‐sparing effect of estrogen are identified by using the microarray technique. Expand
Estrogen receptor specificity for the effects of estrogen in ovariectomized mice.
TLDR
It was found that estrogen increased the cortical bone dimensions in both wild-type (WT) and double ER knockout (DERKO) mice, and four genes that were regulated by estrogen in both WT and DERKO mice were identified. Expand
Elucidation of CPX‐1 involvement in RANKL‐induced osteoclastogenesis by a proteomics approach
TLDR
The transient up‐regulation pattern of CPX‐1 expression may be important for the successful progression from pre‐osteoclasts to mature osteoclasts and led to the inhibition of osteoclast formation, but not pre-osteoclast generation. Expand
Proteome analysis of secreted proteins during osteoclast differentiation using two different methods: Two‐dimensional electrophoresis and isotope‐coded affinity tags analysis with two‐dimensional chromatography
TLDR
Novel proteins related to osteoclast differentiation which are potential therapeutic targets for the treatment of bone diseases, such as osteoporosis are indicated. Expand
A comparison of selected mRNA and protein abundances in human liver
TLDR
A comparison with published data on the abundances of liver mRNAs for plasma proteins (secreted by the liver) suggests that higher abundance messages are strongly enriched in secreted sequences, and the data confirms this. Expand
Estrogen Receptor (ER)-β Reduces ERα-Regulated Gene Transcription, Supporting a “Ying Yang” Relationship between ERα and ERβ in Mice
TLDR
In vivo data indicate that an important physiological role of ERbeta is to modulate ERalpha-mediated gene transcription supporting a "Ying Yang" relationship between ERalpha and ERbeta in mice. Expand
Identification of genes regulated during osteoblastic differentiation by genome-wide expression analysis of mouse calvaria primary osteoblasts in vitro.
TLDR
The expression profiles of 601 regulated genes, classified in distinct functional families, are presented and analyzed here and will aid in the identification of genes that play a significant role in osteoblasts. Expand
The mechanisms of estrogen regulation of bone resorption.
  • B. Riggs
  • Biology, Medicine
  • The Journal of clinical investigation
  • 2000
TLDR
The authors show that ovariectomy-induced bone loss can be prevented by administering either E, T NF-α binding protein, or an inactivating antibody specific for TNF-α, and that bone loss does not occur in OVX, T cell–deficient animals. Expand
Calcyclin, a Ca2+ Ion-binding Protein, Contributes to the Anabolic Effects of Simvastatin on Bone*
TLDR
Calcyclin is one of the candidate proteins that plays a role in osteoblastogenesis in response to simvastatin, although the precise functions of calcyclin in osteoblasts remain to be verified. Expand
Transgenic mice expressing soluble tumor necrosis factor-receptor are protected against bone loss caused by estrogen deficiency.
To evaluate the role of tumor necrosis factor (TNF alpha) in bone loss resulting from estrogen deficiency, the effects of ovariectomy were explored in six-month-old transgenic mice expressing highExpand
...
1
2
3
4
5
...