Proteome, phosphoproteome, and N-glycoproteome are quantitatively preserved in formalin-fixed paraffin-embedded tissue and analyzable by high-resolution mass spectrometry.

@article{Ostasiewicz2010ProteomePA,
  title={Proteome, phosphoproteome, and N-glycoproteome are quantitatively preserved in formalin-fixed paraffin-embedded tissue and analyzable by high-resolution mass spectrometry.},
  author={Paweł Ostasiewicz and Dorota F. Zielinska and Matthias Mann and Jacek R. Wisniewski},
  journal={Journal of proteome research},
  year={2010},
  volume={9 7},
  pages={3688-700}
}
Tissue samples in biobanks are typically formalin-fixed and paraffin-embedded (FFPE), in which form they are preserved for decades. It has only recently been shown that proteins in FFPE tissues can be identified by mass spectrometry-based proteomics but analysis of post-translational modifications is thought to be difficult or impossible. The filter aided sample preparation (FASP) method can analyze proteomic samples solubilized in high concentrations of SDS and we use this feature to develop a… CONTINUE READING
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